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Virus-Induced Gene Silencing in Diploid and Tetraploid Potato Species

  • Jinping Zhao
  • Haolang Jiang
  • Guanyu Wang
  • Zonghua Wang
  • Jingao Dong
  • Junqi SongEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2172)

Abstract

Potato is the world’s fourth largest food crop and a vegetatively propagated model polyploid plant. To facilitate genomic studies in potato, here we describe detailed protocols to silence genes in both diploid potato Solanum bulbocastanum and tetraploid potato cultivars such as Maris Bard, Arran Pilot, Ancilla, and Serrana using tobacco rattle virus (TRV)- or potato virus X (PVX)-induced gene silencing (VIGS) system, respectively. The established VIGS system represents an efficient and powerful approach for functional analysis of genes involved in growth, development, metabolism, and responses to biotic and abiotic stresses in potato.

Key words

Virus-induced gene silencing (VIGS) Diploid potato Tetraploid potato Tobacco rattle virus (TRV) Potato virus X (PVX) Solanum bulbocastanum Solanum tuberosum 

Notes

Acknowledgments

We thank the NRSP-6 US Potato Genebank for providing the wild and cultivated potato species and Dr. Yule Liu from Tsinghua University for providing the TRV1 and TRV2e vectors. This work was supported by a startup fund from the Texas A&M AgriLife Research and the Hatch Project TEX0-1-9675 from USDA National Institute of Food and Agriculture to JS, the scholarship 201707877008 from China Scholarship Council to HJ, and the scholarship 201708130105 from China Scholarship Council to GW.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Jinping Zhao
    • 1
  • Haolang Jiang
    • 1
    • 2
  • Guanyu Wang
    • 3
  • Zonghua Wang
    • 2
    • 4
  • Jingao Dong
    • 3
  • Junqi Song
    • 1
    • 5
    Email author
  1. 1.Texas A&M AgriLife Research Center at DallasTexas A&M University SystemDallasUSA
  2. 2.State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhouChina
  3. 3.Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Mycotoxin and Molecular Plant Pathology Laboratory, College of Life SciencesHebei Agricultural UniversityBaodingChina
  4. 4.Institute of OceanographyMinjiang UniversityFuzhouChina
  5. 5.Department of Plant Pathology & MicrobiologyTexas A&M UniversityCollege StationUSA

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