ALSV-Based Virus-Induced Gene Silencing in Apple Tree (Malus × domestica L.)
- 66 Downloads
Virus-induced gene silencing (VIGS) is a fast and efficient tool to investigate gene function in plant as an alternative to knock down/out transgenic lines, especially in plant species difficult to transform and challenging to regenerate such as perennial woody plants. In apple tree, a VIGS vector has been previously developed based on the Apple latent spherical virus (ALSV) and an efficient inoculation method has been optimized using biolistics. This report described detailed step-by-step procedure to design and silence a gene of interest (GOI) in apple tree tissues using the ALSV-based vector.
Key wordsALSV VIGS Apple tree (Malus × domestica L.) Quinoa (Chenopodium quinoa) Rub-inoculation Biolistics
- 8.Igarashi A, Yamagata K, Sugai T, Takahashi Y, Sugawara E, Tamura A, Yaegashi H, Yamagishi N, Takahashi T, Isogai M, Takahashi H, Yoshikawa N (2009) Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes. Virology 386:407–416CrossRefGoogle Scholar
- 12.Navarro Gallon SM, Elejalde-Palmett C, Daudu D, Liesecke F, Jullien F, Papon N, Dugé de Bernonville T, Courdavault V, Lanoue A, Oudin A, Glévarec G, Pichon O, Clastre M, St-Pierre B, Atehortua L, Yoshikawa N, Giglioli-Guivarc’h N, Besseau S (2017) Virus-induced gene silencing of the two squalene synthase isoforms of apple tree (Malus × domestica L.) negatively impacts phytosterol biosynthesis, plastid pigmentation and leaf growth. Planta 246:45–60CrossRefGoogle Scholar