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ALSV-Based Virus-Induced Gene Silencing in Apple Tree (Malus × domestica L.)

  • Carolina Werner Ribeiro
  • Thomas Dugé de Bernonville
  • Gaëlle Glévarec
  • Arnaud Lanoue
  • Audrey Oudin
  • Olivier Pichon
  • Benoit St-Pierre
  • Vincent Courdavault
  • Sébastien BesseauEmail author
Protocol
  • 66 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2172)

Abstract

Virus-induced gene silencing (VIGS) is a fast and efficient tool to investigate gene function in plant as an alternative to knock down/out transgenic lines, especially in plant species difficult to transform and challenging to regenerate such as perennial woody plants. In apple tree, a VIGS vector has been previously developed based on the Apple latent spherical virus (ALSV) and an efficient inoculation method has been optimized using biolistics. This report described detailed step-by-step procedure to design and silence a gene of interest (GOI) in apple tree tissues using the ALSV-based vector.

Key words

ALSV VIGS Apple tree (Malus × domestica L.) Quinoa (Chenopodium quinoaRub-inoculation Biolistics 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Carolina Werner Ribeiro
    • 1
  • Thomas Dugé de Bernonville
    • 1
  • Gaëlle Glévarec
    • 1
  • Arnaud Lanoue
    • 1
  • Audrey Oudin
    • 1
  • Olivier Pichon
    • 1
  • Benoit St-Pierre
    • 1
  • Vincent Courdavault
    • 1
  • Sébastien Besseau
    • 1
    Email author
  1. 1.EA2106 Biomolécules et Biotechnologies VégétalesUniversité de ToursToursFrance

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