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Caveolae pp 63-70 | Cite as

Spatiotemporal Analysis of Caveolae Dynamics Using Total Internal Reflection Fluorescence Microscopy

  • Yosuke SenjuEmail author
  • Shiro SuetsuguEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2169)

Abstract

Total internal reflection fluorescence microscopy enables to analyze the localizations and dynamics of cellular events that occur at or near the plasma membrane. Total internal reflection fluorescence microscopy exclusively illuminates molecules in the close vicinity of the glass surface, thereby reducing background fluorescence and enabling observation of the plasma membrane in the glass-attached cells with a high signal-to-noise ratio. Here, we describe the application of total internal reflection fluorescence microscopy to analyze the dynamics of caveolae, which play essential physiological functions, including membrane tension buffering, endocytosis, and signaling at the plasma membrane.

Key words

Total internal reflection fluorescence microscopy Kymograph Endocytosis Caveolae Lateral diffusion 

Notes

Acknowledgments

This work was supported by grants from the Funding Program for Next Generation World Leading Researchers (NEXT program) (grant number: LS031); Japan Society for the Promotion of Science (JSPS) KAKENHI (grant numbers: 26291037, 15H01641, 15H05902). Astellas Foundation for Research on Metabolic Disorders to S.S.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Research Institute for Interdisciplinary Science (RIIS)Okayama UniversityOkayamaJapan
  2. 2.Division of Biological ScienceNara Institute of Science and TechnologyNaraJapan

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