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Caveolae pp 1-10 | Cite as

Selective Visualization of Caveolae by TEM Using APEX2

  • Alexander LudwigEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2169)

Abstract

Caveolae are small flask- or cup-shaped invaginations of the plasma membrane found in almost all vertebrate cells. Due to their small size (50–100 nm), transmission electron microscopy (TEM) has been the method of choice to study caveolae formation and ultrastructure and, more recently, to resolve the sub-caveolar localization of its protein components using novel protein labeling methods for TEM. This chapter describes a protocol for the selective visualization of caveolae and caveolar proteins by TEM, 3D tomography, and correlative light and electron microscopy (CLEM) using the peroxidase APEX2.

Key words

Caveolae APEX2 TEM 3D tomography Correlative light and electron microscopy Caveolin Cavin 

Notes

Acknowledgments

This work was supported by grants from MOE Singapore (TIER1 RG39-14) and Nanyang Technological University (NIM/03/2016).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.School of Biological Sciences, NTU Institute of Structural BiologyNanyang Technological UniversitySingaporeSingapore

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