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Kupffer Cells pp 129-143 | Cite as

A Transposon-Based Mouse Model of Hepatocellular Carcinoma via Hydrodynamic Tail Vein Injection

  • Sijia Yu
  • Santiago VerniaEmail author
Protocol
  • 45 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2164)

Abstract

Transgenic mouse are reliable, convenient models for studying human hepatocellular carcinoma (HCC). The development of a synthetically engineered Sleeping Beauty (SB) transposon system further enables the viral-free, efficient delivery of desired oncogenes to mouse tissues. Here, we describe an SB transposon-based approach to induce HCC in mice by expressing a hyperactive form of N-RAS, N-RASG12V, while silencing the endogenous Trp53 gene via hydrodynamic tail vein injection, a method to rapidly deliver naked plasmids to mouse liver.

Key words

Hepatocellular carcinoma Mouse tumor model N-RAS p53 Sleeping Beauty Hydrodynamic tail vein injection 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.MRC London Institute of Medical Sciences, Hammersmith Hospital CampusLondonUK
  2. 2.Institute of Clinical SciencesFaculty of Medicine, Imperial College LondonLondonUK

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