Advertisement

Kupffer Cells pp 101-108 | Cite as

Enzymatic Quantification of Liver Lipids After Folch Extraction

  • Mirko E. MinnitiEmail author
  • Osman Ahmed
  • Matteo PedrelliEmail author
Protocol
  • 42 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2164)

Abstract

The determination of the lipid content in liver offers the potential to investigate metabolic alterations in different research contexts. Here, we describe a method to determine cholesterol, triglycerides, and phospholipids in liver samples based on total lipid isolation by a 2:1 chloroform–methanol mixture (Folch extraction) and specific enzymatic colorimetric microassays in plate.

Key words

Liver Cholesterol Triglycerides Phospholipids Enzymatic assay 

References

  1. 1.
    Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226(1):497–509PubMedGoogle Scholar
  2. 2.
    Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911–917.  https://doi.org/10.1139/o59-099CrossRefGoogle Scholar
  3. 3.
    Carr TP, Andresen CJ, Rudel LL (1993) Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts. Clin Biochem 26(1):39–42CrossRefGoogle Scholar
  4. 4.
    Allain CC, Poon LS, Chan CS, Richmond W, Fu PC (1974) Enzymatic determination of total serum cholesterol. Clin Chem 20(4):470–475CrossRefGoogle Scholar
  5. 5.
    Siedel J, Schmuck R, Staepels J, Town MH (1993) Long term stable liquid ready-to-use monoreagent for the enzymatic assay of serum or plasma triglycerides (GPO-PAP method). Clin Chem 39(6):1127Google Scholar
  6. 6.
    Takayama M, Itoh S, Nagasaki T, Tanimizu I (1977) A new enzymatic method for determination of serum choline-containing phospholipids. Clin Chim Acta 79(1):93–98CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Division of Clinical Chemistry, Department of Laboratory MedicineKarolinska InstitutetStockholmSweden

Personalised recommendations