A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli
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Mammalian DSPs have been historically isolated either from native tissue sources or from transfected insect cell cultures via time-consuming and cumbersome protocols often yielding protein of variable quality and quantity. A facile and highly reproducible alternative methodology involving the heterologous expression and purification of soluble mammalian DSPs from E. coli, which yields highly active and functional protein of a uniform quality and quantity, free of spurious posttranslational modifications inherent to mammalian and insect cell expression systems, is described in this chapter.
Key wordsDSP Purification Escherichia coli Polyhistidine Tag Dynamin Drp1 OPA1
This work was supported by National Institutes of Health grant R01GM121583 awarded to R. R.