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Quantifying Drp1-Mediated Mitochondrial Fission by Immunostaining in Fixed Cells

  • Di Hu
  • Xin QiEmail author
Protocol
  • 85 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2159)

Abstract

Dynamin-like protein 1 (Drp1) is the master regulator of mitochondrial fission. Drp1 translocates from the cytosol to the mitochondrial outer membrane to execute the scission process. Here we describe an immunofluorescence-based method to measure the mitochondrial translocation of Drp1 and quantify Drp1-related mitochondrial fission by labeling the mitochondrial import receptor subunit TOM20 in fixed cell culture.

Key words

Mitochondrial fission Dynamin-related protein 1 TOM20 Immunofluorescence staining Confocal microscopy 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Departments of Physiology & BiophysicsCase Western Reserve University School of MedicineClevelandUSA
  2. 2.Center for Mitochondrial DiseasesCase Western Reserve University School of MedicineClevelandUSA

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