Quantifying Drp1-Mediated Mitochondrial Fission by Immunostaining in Fixed Cells
- 85 Downloads
Dynamin-like protein 1 (Drp1) is the master regulator of mitochondrial fission. Drp1 translocates from the cytosol to the mitochondrial outer membrane to execute the scission process. Here we describe an immunofluorescence-based method to measure the mitochondrial translocation of Drp1 and quantify Drp1-related mitochondrial fission by labeling the mitochondrial import receptor subunit TOM20 in fixed cell culture.
Key wordsMitochondrial fission Dynamin-related protein 1 TOM20 Immunofluorescence staining Confocal microscopy
- 2.Chan DC (2006) Mitochondrial fusion and fission in mammals. Annu Rev Cell Dev Biol 22:79–99. https://doi.org/10.1146/annurev.cellbio.22.010305.104638CrossRefPubMedGoogle Scholar