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Measuring Drp1 Activity in Mitochondrial Fission In Vivo

  • Di Hu
  • Xin QiEmail author
Protocol
  • 76 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2159)

Abstract

Mitochondrial fission is mainly regulated by a number of dynamin superfamily proteins or DSPs, of which dynamin-like protein 1 (Drp1) is responsible for the scission process during mitochondrial fission. Here we describe several methods, including monitoring mitochondrial distribution, phosphorylation, and tetramer level of Drp1, to examine the activity of Drp1 in mitochondrial fission in vivo.

Key words

Mitochondrial dynamics Dynamin-related protein 1 Phosphorylation Western blotting Tetramerization 

Notes

Acknowledgment

This work was supported by NIH R01 NS088192 to X.Q.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Departments of Physiology & BiophysicsCase Western Reserve University School of MedicineClevelandUSA
  2. 2.Center for Mitochondrial DiseasesCase Western Reserve University School of MedicineClevelandUSA

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