Mitochondrial fission is mainly regulated by a number of dynamin superfamily proteins or DSPs, of which dynamin-like protein 1 (Drp1) is responsible for the scission process during mitochondrial fission. Here we describe several methods, including monitoring mitochondrial distribution, phosphorylation, and tetramer level of Drp1, to examine the activity of Drp1 in mitochondrial fission in vivo.
Mitochondrial dynamics Dynamin-related protein 1 Phosphorylation Western blotting Tetramerization
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This work was supported by NIH R01 NS088192 to X.Q.
Gottlieb RA (2000) Role of mitochondria in apoptosis. Crit Rev Eukaryot Gene Expr 10(3-4):231–239CrossRefGoogle Scholar
Chan DC (2006) Mitochondria: dynamic organelles in disease, aging, and development. Cell 125(7):1241–1252CrossRefGoogle Scholar
Chan DC (2006) Mitochondrial fusion and fission in mammals. Annu Rev Cell Dev Biol 22:79–99CrossRefGoogle Scholar
Fannjiang Y et al (2004) Mitochondrial fission proteins regulate programmed cell death in yeast. Genes Dev 18(22):2785–2797CrossRefGoogle Scholar
James DI et al (2003) hFis1, a novel component of the mammalian mitochondrial fission machinery. J Biol Chem 278(38):36373–36379CrossRefGoogle Scholar
Taguchi N et al (2007) Mitotic phosphorylation of dynamin-related GTPase Drp1 participates in mitochondrial fission. J Biol Chem 282(15):11521–11529CrossRefGoogle Scholar
Chang CR, Blackstone C (2007) Cyclic AMP-dependent protein kinase phosphorylation of Drp1 regulates its GTPase activity and mitochondrial morphology. J Biol Chem 282(30):21583–21587CrossRefGoogle Scholar
Cribbs JT, Strack S (2007) Reversible phosphorylation of Drp1 by cyclic AMP-dependent protein kinase and calcineurin regulates mitochondrial fission and cell death. EMBO Rep 8(10):939–944CrossRefGoogle Scholar
Qi X et al (2013) A novel Drp1 inhibitor diminishes aberrant mitochondrial fission and neurotoxicity. J Cell Sci 126(Pt 3):789–802CrossRefGoogle Scholar
Roe AJ, Qi X (2018) Drp1 phosphorylation by MAPK1 causes mitochondrial dysfunction in cell culture model of Huntington’s disease. Biochem Biophys Res Commun 496(2):706–711CrossRefGoogle Scholar