Silicone Chambers for Pollen Tube Imaging in Microstructured In Vitro Environments
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Live cell imaging at high resolution of pollen tubes growing in vitro requires an experimental setup that maintains the elongated cells in a single optical plane and allows for controlled exchange of growth medium. As a low-cost alternative to lithography-based microfluidics, we developed a silicone-based spacer system that allows introducing spatial features and flexible design. These growth chambers can be cleaned and reused repeatedly.
Key wordsLive cell imaging Fluorescence imaging Micromanipulation Silicone chambers
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