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Galvanotropic Chamber for Controlled Reorientation of Pollen Tube Growth and Simultaneous Confocal Imaging of Intracellular Dynamics

  • Firas Bou Daher
  • Anja GeitmannEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2160)

Abstract

Successful fertilization and seed set require the pollen tube to grow through several tissues, to change its growth orientation by responding to directional cues, and to ultimately reach the embryo sac and deliver the paternal genetic material. The ability to respond to external directional cues is, therefore, a pivotal feature of pollen tube behavior. In order to study the regulatory mechanisms controlling and mediating pollen tube tropic growth, a robust and reproducible method for the induction of growth reorientation in vitro is required. Here we describe a galvanotropic chamber designed to expose growing pollen tubes to precisely calibrated directional cues triggering reorientation while simultaneously tracking subcellular processes using live cell imaging and confocal laser scanning microscopy.

Key words

Directional cues Galvanotropism Galvanotropic chamber Guided growth Pollen tube Tropic growth 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Plant and Microbial BiologyUniversity of MinnesotaSaint PaulUSA
  2. 2.Department of Plant ScienceMcGill UniversityMontrealCanada

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