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A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

  • Yu Song
  • Hieu Sy Vu
  • Sunitha Shiva
  • Carl Fruehan
  • Mary R. Roth
  • Pamela Tamura
  • Ruth WeltiEmail author
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 2156)

Abstract

Lipid changes that occur in leaves of plants (e.g., Arabidopsis thaliana), during cold and freezing stress can be analyzed with electrospray ionization triple quadrupole mass spectrometry, using high-throughput multiple reaction monitoring (MRM). An online tool, LipidomeDB Data Calculation Environment, is employed for mass spectral data processing.

Key words

Cold Freezing Postfreezing recovery Lipidomics Mass spectrometry Multiple reaction monitoring (MRM) Lipidomics data processing 

Notes

Acknowledgments

The authors would like to thank lab member Libin Yao for her contributions to plant stress experiments in our laboratory, Mark Ungerer for use of his lab’s freezing chamber, and Ari Jumpponen for use of his lab’s light cart. This work was supported by the USDA National Institute of Food and Agriculture, Hatch/Multi-State project 1013013, and National Science Foundation MCB 1413036. Instrument acquisition at KLRC was supported by National Science Foundation (EPS 0236913, DBI 0521587, DBI 1228622, DBI 1726527), K-IDeA Networks of Biomedical Research Excellence (INBRE) of National Institute of Health (P20GM103418), and Kansas State University. Contribution no. 20-008-B from the Kansas Agricultural Experiment Station.

Supplementary material

476740_2_En_14_MOESM1_ESM.xlsx (73 kb)
Supplemental Table 1 Song_et_al_Supplemental tables 1 to 4 (XLSX 74 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Yu Song
    • 1
  • Hieu Sy Vu
    • 1
    • 2
  • Sunitha Shiva
    • 1
    • 3
  • Carl Fruehan
    • 1
  • Mary R. Roth
    • 1
  • Pamela Tamura
    • 1
  • Ruth Welti
    • 1
    Email author
  1. 1.Kansas Lipidomics Research Center, Division of BiologyKansas State UniversityManhattanUSA
  2. 2.Children’s Medical Research Institute at University of Texas-SouthwesternDallasUSA
  3. 3.Department of Biochemistry and Molecular BiophysicsKansas State UniversityManhattanUSA

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