A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition
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Lipid changes that occur in leaves of plants (e.g., Arabidopsis thaliana), during cold and freezing stress can be analyzed with electrospray ionization triple quadrupole mass spectrometry, using high-throughput multiple reaction monitoring (MRM). An online tool, LipidomeDB Data Calculation Environment, is employed for mass spectral data processing.
Key wordsCold Freezing Postfreezing recovery Lipidomics Mass spectrometry Multiple reaction monitoring (MRM) Lipidomics data processing
The authors would like to thank lab member Libin Yao for her contributions to plant stress experiments in our laboratory, Mark Ungerer for use of his lab’s freezing chamber, and Ari Jumpponen for use of his lab’s light cart. This work was supported by the USDA National Institute of Food and Agriculture, Hatch/Multi-State project 1013013, and National Science Foundation MCB 1413036. Instrument acquisition at KLRC was supported by National Science Foundation (EPS 0236913, DBI 0521587, DBI 1228622, DBI 1726527), K-IDeA Networks of Biomedical Research Excellence (INBRE) of National Institute of Health (P20GM103418), and Kansas State University. Contribution no. 20-008-B from the Kansas Agricultural Experiment Station.