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Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins

  • Md Mostafa Kamal
  • Daisuke Takahashi
  • Takato Nakayama
  • Yushi Miki
  • Yukio Kawamura
  • Matsuo UemuraEmail author
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 2156)

Abstract

Plasma membrane is the primary determinant of freezing tolerance in plants because of its central role in freeze–thaw cycle. Changes in plasma membrane protein composition have been one of the major research areas in plant cold acclimation. To obtain comprehensive profiles of the plasma membrane proteomes and their changes during the cold acclimation process, a plasma membrane purification method using a dextran–polyethylene glycol two polymer system and a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for the plasma membrane proteins are described. The proteomic results obtained are further applied to label-free protein semiquantification.

Key words

Cold acclimation Plasma membrane Nano-LC-MS/MS Shotgun proteomics Label-free semiquantification In-solution digestion In-gel digestion 

Notes

Acknowledgments

This work was supported in part by a Research Fellowship for Young Scientists (#247373 to D.T.) and Grants-in-Aid for Scientific Research (#22120003, #24370018, #17H03961 to M.U. and Y.K.) from JSPS, Japan.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Md Mostafa Kamal
    • 1
  • Daisuke Takahashi
    • 1
    • 2
  • Takato Nakayama
    • 3
  • Yushi Miki
    • 3
  • Yukio Kawamura
    • 1
    • 3
  • Matsuo Uemura
    • 1
    • 3
    Email author
  1. 1.United Graduate School of Agricultural Sciences, Faculty of AgricultureIwate UniversityMoriokaJapan
  2. 2.Graduate School of Science & EngineeringSaitama UniversitySaitamaJapan
  3. 3.Department of Plant-Bioscience, Faculty of AgricultureIwate UniversityMoriokaJapan

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