Fabrication of Corneal Extracellular Matrix-Derived Hydrogels
- 49 Downloads
Hydrogels derived from corneal extracellular matrix (ECM) represent a promising biomaterial for corneal repair and regeneration. To fabricate these hydrogels, first corneas need to be decellularized using repeated freeze-thaw cycles and nucleases to remove all nuclear and cellular components. The remaining corneal ECM is lyophilized to remove all water and milled into a fine powder. The ECM powder is weighed and dissolved in pepsin solution at a concentration of 20 mg/mL. Hydrogels are formed by neutralizing the pH of the solution and maintaining it at 37 °C until fibrillogenesis has occurred. Corneal stromal cells may be suspended throughout the hydrogel solution prior to gelation to generate a corneal stromal substitute.
Key wordsHydrogel Scaffold Cornea Stroma Collagen Extracellular matrix Keratocyte
The research is supported by funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 637460) and Science Foundation Ireland and Marie-Curie Action COFUND (grant no. 11/SIRG/B2104).
- 15.Kim H, Jang J, Park J, Lee KP, Lee S, Lee DM, Kim KH, Kim HK, Cho DW (2019) Shear-induced alignment of collagen fibrils using 3d cell printing for corneal stroma tissue engineering. Biofabrication 11(3). https://doi.org/10.1088/1758-5090/ab1a8b
- 17.Fernandez-Perez J, Ahearne M (2019) Decellularization and recellularization of cornea: progress towards a donor alternative. Methods 171:86–96. https://doi.org/10.1016/j.ymeth.2019.05.009
- 19.Crapo PM, Gilbert TW, Badylak SF (2011) An overview of tissue and whole organ decellularization processes. Biomaterials 32(12):3233–3243. https://doi.org/10.1016/j.biomaterials.2011.01.057CrossRefPubMedPubMedCentralGoogle Scholar