Aging pp 57-65 | Cite as

Mouse Fitness as Determined Through Treadmill Running and Walking

  • Joseph C. Reynolds
  • Changhan LeeEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2144)


Endurance testing simultaneously assesses a wide variety of physiological systems including the cardiovascular, respiratory, metabolic, and neuromuscular systems (Gabriel and Zierath, Cell Metab 25:1000–1011, 2017). Treadmill running is a noninvasive method to evaluate fitness capacity in a longitudinal or cross-sectional manner. High-intensity exercise tests can be used to determine peak physical capacity in mice. However, because aging is associated with a progressive loss of physical capacity the running protocols can be adapted and optimized for aged mice.

Key words

Mice Fitness Running Treadmill Aging 


  1. 1.
    Gabriel BM, Zierath JR (2017) The limits of exercise physiology: from performance to health. Cell Metab 25(5):1000–1011. Scholar
  2. 2.
    Richardson A, Fischer KE, Speakman JR, de Cabo R, Mitchell SJ, Peterson CA, Rabinovitch P, Chiao YA, Taffet G, Miller RA, Renteria RC, Bower J, Ingram DK, Ladiges WC, Ikeno Y, Sierra F, Austad SN (2016) Measures of healthspan as indices of aging in mice—a recommendation. J Gerontol A Biol Sci Med Sci 71(4):427–430. Scholar
  3. 3.
    Dougherty JP, Springer DA, Gershengorn MC (2016) The treadmill fatigue test: a simple, high-throughput assay of fatigue-like behavior for the mouse. J Vis Exp (111).
  4. 4.
    Conner JD, Wolden-Hanson T, Quinn LS (2014) Assessment of murine exercise endurance without the use of a shock grid: an alternative to forced exercise. J Vis Exp (90):e51846.
  5. 5.
    Castro B, Kuang S (2017) Evaluation of muscle performance in mice by treadmill exhaustion test and whole-limb grip strength assay. Bio Protoc 7(8).
  6. 6.
    Batra A, Vohra RS, Chrzanowski SM, Hammers DW, Lott DJ, Vandenborne K, Walter GA, Forbes SC (2019) Effects of PDE5 inhibition on dystrophic muscle following an acute bout of downhill running and endurance training. J Appl Physiol (1985) 126(6):1737–1745. Scholar
  7. 7.
    Feng R, Wang L, Li Z, Yang R, Liang Y, Sun Y, Yu Q, Ghartey-Kwansah G, Sun Y, Wu Y, Zhang W, Zhou X, Xu M, Bryant J, Yan G, Isaacs W, Ma J, Xu X (2019) A systematic comparison of exercise training protocols on animal models of cardiovascular capacity. Life Sci 217:128–140. Scholar
  8. 8.
    Hollinski R, Osterberg A, Polei S, Lindner T, Cantre D, Mittlmeier T, Vollmar B, Bruhn S, Muller-Hilke B (2018) Young and healthy C57BL/6 J mice performing sprint interval training reveal gender- and site-specific changes to the cortical bone. Sci Rep 8(1):1529. Scholar
  9. 9.
    Mees LM, Coulter MM, Chrenek MA, Motz CT, Landis EG, Boatright JH, Pardue MT (2019) Low-intensity exercise in mice is sufficient to protect retinal function during light-induced retinal degeneration. Invest Ophthalmol Vis Sci 60(5):1328–1335. Scholar
  10. 10.
    Walton RD, Jones SA, Rostron KA, Kayani AC, Close GL, McArdle A, Lancaster MK (2016) Interactions of short-term and chronic treadmill training with aging of the left ventricle of the heart. J Gerontol A Biol Sci Med Sci 71(8):1005–1013. Scholar
  11. 11.
    Nguemeni C, McDonald MW, Jeffers MS, Livingston-Thomas J, Lagace D, Corbett D (2018) Short- and long-term exposure to low and high dose running produce differential effects on hippocampal neurogenesis. Neuroscience 369:202–211. Scholar
  12. 12.
    Hoene M, Li J, Li Y, Runge H, Zhao X, Haring HU, Lehmann R, Xu G, Weigert C (2016) Muscle and liver-specific alterations in lipid and acylcarnitine metabolism after a single bout of exercise in mice. Sci Rep 6:22218. Scholar
  13. 13.
    Shi Y, Shi H, Nieman DC, Hu Q, Yang L, Liu T, Zhu X, Wei H, Wu D, Li F, Cui Y, Chen P (2019) Lactic acid accumulation during exhaustive exercise impairs release of neutrophil extracellular traps in mice. Front Physiol 10:709. Scholar
  14. 14.
    Ikeda SI, Tamura Y, Kakehi S, Sanada H, Kawamori R, Watada H (2016) Exercise-induced increase in IL-6 level enhances GLUT4 expression and insulin sensitivity in mouse skeletal muscle. Biochem Biophys Res Commun 473(4):947–952. Scholar
  15. 15.
    Brandt N, Dethlefsen MM, Bangsbo J, Pilegaard H (2017) PGC-1alpha and exercise intensity dependent adaptations in mouse skeletal muscle. PLoS One 12(10):e0185993. Scholar
  16. 16.
    Sako H, Yada K, Suzuki K (2016) Genome-wide analysis of acute endurance exercise-induced translational regulation in mouse skeletal muscle. PLoS One 11(2):e0148311. Scholar
  17. 17.
    Jin Q, Qiao C, Li J, Xiao B, Li J, Xiao X (2019) A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice. Skelet Muscle 9(1):16. Scholar
  18. 18.
    Gill JF, Santos G, Schnyder S, Handschin C (2018) PGC-1alpha affects aging-related changes in muscle and motor function by modulating specific exercise-mediated changes in old mice. Aging Cell 17(1):e12697. Scholar
  19. 19.
    Das A, Huang GX, Bonkowski MS, Longchamp A, Li C, Schultz MB, Kim LJ, Osborne B, Joshi S, Lu Y, Trevino-Villarreal JH, Kang MJ, Hung TT, Lee B, Williams EO, Igarashi M, Mitchell JR, Wu LE, Turner N, Arany Z, Guarente L, Sinclair DA (2018) Impairment of an endothelial NAD(+)-H2S signaling network is a reversible cause of vascular aging. Cell 173(1):74–89. e20. Scholar
  20. 20.
    Moore KM, Girens RE, Larson SK, Jones MR, Restivo JL, Holtzman DM, Cirrito JR, Yuede CM, Zimmerman SD, Timson BF (2016) A spectrum of exercise training reduces soluble Abeta in a dose-dependent manner in a mouse model of Alzheimer’s disease. Neurobiol Dis 85:218–224. Scholar
  21. 21.
    Tahara Y, Shibata S (2018) Entrainment of the mouse circadian clock: effects of stress, exercise, and nutrition. Free Radic Biol Med 119:129–138. Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Leonard Davis School of GerontologyUniversity of Southern CaliforniaLos AngelesUSA
  2. 2.USC Norris Comprehensive Cancer CenterLos AngelesUSA
  3. 3.Biomedical Science, Graduate SchoolAjou UniversitySuwonSouth Korea

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