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3D Bioprinting pp 251-258 | Cite as

Bioprinting 3D Human Induced Pluripotent Stem Cell Constructs for Multilineage Tissue Engineering and Modeling

  • Jeremy M. CrookEmail author
  • Eva Tomaskovic-CrookEmail author
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Part of the Methods in Molecular Biology book series (MIMB, volume 2140)

Abstract

Bioprinting human pluripotent stem cells (PSCs) provides an opportunity to produce three-dimensional (3D) cell-laden constructs with the potential to be differentiated in vitro to all tissue types of the human body. Here, we detail a previously published method for 3D printing human induced pluripotent stem cells (iPSCs; also applicable to human embryonic stem cells) within a clinically amenable bioink (also described in Chapter  10) that is cross-linked to a 3D construct. The printed iPSCs continue to have self-replicating and multilineage cell induction potential in situ, and the constructs are robust and amenable to different differentiation protocols for fabricating diverse tissue types, with the potential to be applied for both research- and clinical-product development.

Key words

3D bioprinting Clinically amenable bioink Human induced pluripotent stem cells Multilineage Tissue 

Notes

Acknowledgment

The authors wish to acknowledge funding from the Australian Research Council (ARC) Centre of Excellence Scheme (CE140100012).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM FacilityUniversity of WollongongWollongongAustralia
  2. 2.Illawarra Health and Medical Research InstituteUniversity of WollongongWollongongAustralia
  3. 3.Department of Surgery, St Vincent’s HospitalThe University of MelbourneFitzroyAustralia

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