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3D Bioprinting pp 217-228 | Cite as

Bioprinting for Skin

  • Cristina Quílez
  • Gonzalo de Aranda Izuzquiza
  • Marta García
  • Verónica López
  • Andrés Montero
  • Leticia Valencia
  • Diego VelascoEmail author
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 2140)

Abstract

We describe an extrusion-based method to print a human bilayered skin using bioinks containing human plasma and primary human fibroblasts and keratinocytes from skin biopsies. We generate 100 cm2 of printed skin in less than 35 min. We analyze its structure using histological and immunohistochemical methods, both in in vitro 3D cultures and upon transplantation to immunodeficient mice. We have demonstrated that the printed skin is similar to normal human skin and indistinguishable from bilayered dermo-epidermal equivalents, previously produced manually in our laboratory and successfully used in the clinic.

Key words

3D bioprinting Skin bioprinting Skin tissue engineering Artificial skin Skin equivalents 3D skin culture Fibrin hydrogel 

Notes

Acknowledgments

We kindly thank Prof. José Luis Jorcano (from UC3M) and Prof. Juan F. del Cañizo (Hospital General Universitario Gregorio Marañón) for their guidance and support. This work was supported by Programa de Actividades de I+D entre Grupos de Investigación de la Comunidad de Madrid, S2018/BAA-4480, Biopieltec-CM, Programa Estatal de I+D+i Orientada a los Retos de la Sociedad, RTI2018-101627-B-I00 and Cátedra Fundación Ramón Areces. The authors declare no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Cristina Quílez
    • 1
  • Gonzalo de Aranda Izuzquiza
    • 1
  • Marta García
    • 1
    • 2
    • 3
  • Verónica López
    • 2
  • Andrés Montero
    • 1
  • Leticia Valencia
    • 1
  • Diego Velasco
    • 1
    Email author
  1. 1.Department of Bioengineering & Aerospace EngineeringUniversidad Carlos III de Madrid (UC3M)MadridSpain
  2. 2.Division of Epithelial BiomedicineCIEMAT-CIBERERMadridSpain
  3. 3.Department of Basic ResearchInstituto de Investigación Sanitaria de la Fundación Jiménez DíazMadridSpain

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