Testing the Effects of Dietary Seafood Consumption on Depressive Symptoms

  • Maximus Berger
  • G. Paul Amminger
  • Robyn McDermott
  • Paul C. Guest
  • Zoltán SarnyaiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2138)


This chapter presents a protocol for assessing the effects dietary seafood consumption on depressive symptoms. We designed a cross-sectional study of 206 participants recruited in two Torres Strait Island communities. Depressive symptoms were assessed using the adapted Patient Health Questionnaire-9 (aPHQ-9), diet was analyzed with a structured questionnaire, omega-3 and omega-6 fatty acid concentrations were measured via a capillary dried blood spot system, and plasma levels of triglycerides and cholesterol were measured by gas-phase chromatography. Finally, we tested the relationship between seafood consumption, blood lipid concentrations, and depression scores using independent samples t-tests and a logistic and quantile regression model.

Key words

Depression Patient Health Questionnaire-9 Screening Omega-3 fatty acids Omega-6 fatty acids Eicosapentaenoic acid Docosahexaenoic acid 



This work was funded by NHMRC Grant GNT0631947 and by the Centre for Chronic Disease Prevention. We thank all study participants and their families of the Waiben and Mer communities, as well as the Torres and Cape Hospital and Health Service Primary Health Care and Mental Health teams, the Mer Island Primary Health Care Centre team, and JCU medical and dental students.


  1. 1.
    Holub BJ (1988) Dietary fish oils containing eicosapentaenoic acid and the prevention of atherosclerosis and thrombosis. CMAJ 139(5):377–381PubMedPubMedCentralGoogle Scholar
  2. 2.
    Skerrett PJ, Hennekens CH (2003) Consumption of fish and fish oils and decreased risk of stroke. Prev Cardiol 6(1):38–41CrossRefGoogle Scholar
  3. 3.
    Pauwels EK, Volterrani D, Mariani G, Kairemo K (2009) Fatty acid facts, Part IV: docosahexaenoic acid and Alzheimer’s disease. A story of mice, men and fish. Drug News Perspect 22(4):205–213CrossRefGoogle Scholar
  4. 4.
    Szymanski KM, Wheeler DC, Mucci LA (2010) Fish consumption and prostate cancer risk: a review and meta-analysis. Am J Clin Nutr 92(5):1223–1233CrossRefGoogle Scholar
  5. 5.
    Simopoulos AP (2008) The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp Biol Med 233:674–688CrossRefGoogle Scholar
  6. 6.
    Thota RN, Ferguson JJA, Abbott KA, Dias CB, Garg ML (2018) Science behind the cardio-metabolic benefits of omega-3 polyunsaturated fatty acids: biochemical effects vs. clinical outcomes. Food Funct 9(7):3576–3596CrossRefGoogle Scholar
  7. 7.
    Parker G, Gibson NA, Brotchie H, Heruc G, Rees AM, Hadzi-Pavlovic D (2006) Omega-3 fatty acids and mood disorders. Am J Psychiatry 163(6):969–978CrossRefGoogle Scholar
  8. 8.
    Calder PC, Yaqoob P (2009) Omega-3 polyunsaturated fatty acids and human health outcomes. Biofactors 35(3):266–272CrossRefGoogle Scholar
  9. 9.
    Matsuoka YJ, Sawada N, Mimura M, Shikimoto R, Nozaki S, Hamazaki K et al (2017) Dietary fish, n-3 polyunsaturated fatty acid consumption, and depression risk in Japan: a population-based prospective cohort study. Transl Psychiatry 7(9):e1242. Scholar
  10. 10.
    Assies J, Pouwer F, Lok A, Mocking RJ, Bockting CL, Visser I et al (2010) Plasma and erythrocyte fatty acid patterns in patients with recurrent depression: a matched case-control study. PLoS One 5(5):e10635. Scholar
  11. 11.
    Lin PY, Huang SY, Su KP (2010) A meta-analytic review of polyunsaturated fatty acid compositions in patients with depression. Biol Psychiatry 68(2):140–147CrossRefGoogle Scholar
  12. 12.
    McNamara RK, Jandacek R, Rider T, Tso P, Dwivedi Y, Pandey GN (2010) Selective deficits in erythrocyte docosahexaenoic acid composition in adult patients with bipolar disorder and major depressive disorder. J Affect Disord 126(1–2):303–311CrossRefGoogle Scholar
  13. 13.
    McNamara RK (2016) Role of omega-3 fatty acids in the etiology, treatment, and prevention of depression: Current status and future directions. J Nutr Intermed Metab 5:96–106CrossRefGoogle Scholar
  14. 14.
    Hibbeln JR (1998) Fish consumption and major depression. Lancet 351(9110):1213. Scholar
  15. 15.
    Simopoulos AP (2011) Evolutionary aspects of diet: The omega-6/omega-3 ratio and the brain. Mol Neurobiol 44(2):203–215CrossRefGoogle Scholar
  16. 16.
    Brown A, Mentha R, Rowley K, Skinner T, Davy C, O’Dea K (2013) Depression in aboriginal men in central Australia: adaptation of the patient health questionnaire 9. BMC Psychiatry 13(1):271. Scholar
  17. 17.
    Bae JH, Kim G (2018) Systematic review and meta-analysis of omega-3-fatty acids in elderly patients with depression. Nutr Res 50:1–9CrossRefGoogle Scholar
  18. 18.
  19. 19.
  20. 20.
    Taylor S, McDermott R, Thompson F, Usher K (2016) Depression and diabetes in the remote Torres Strait islands. Health Promot J Aust 28(1):59–66CrossRefGoogle Scholar
  21. 21.
    Berger M, Taylor S, Harriss L, Campbell S, Thompson F, Jones S et al (2018) Cross-sectional association of seafood consumption, polyunsaturated fatty acids and depressive symptoms in two Torres Strait communities. Nutr Neurosci 3:1–10. Scholar
  22. 22.
    Liu G, Mühlhäusler BS, Gibson RA (2014) A method for long term stabilisation of long chain polyunsaturated fatty acids in dried blood spots and its clinical application. Prostaglandins Leukot Essent Fat Acids 91(6):251–260CrossRefGoogle Scholar
  23. 23.
    Grosso G, Micek A, Marventano S, Castellano S, Mistretta A, Pajak A et al (2016) Dietary n-3 PUFA, fish consumption and depression: a systematic review and meta-analysis of observational studies. J Affect Disord 205:269–281CrossRefGoogle Scholar
  24. 24.
    Berk M (2004) Dietary omega-3 fatty acids and depression in a community sample. Nutr Neurosci 7(2):101–106CrossRefGoogle Scholar
  25. 25.
    Hoffmire CA, Block RC, Thevenet-Morrison K, van Wijngaarden E (2012) Associations between omega-3 poly-unsaturated fatty acids from fish consumption and severity of depressive symptoms: an analysis of the 2005-2008 National Health and Nutrition Examination Survey. Prostag Leukot Fatty Acids 86(4–5):155–160CrossRefGoogle Scholar
  26. 26.
    Hackett ML, Farnbach S, Glozier N, Skinner T, Teixeira-Pinto A, Askew D et al (2016) Getting it right: Study protocol to determine the diagnostic accuracy of a culturally-specific measure to screen for depression in Aboriginal and/or Torres Strait Islander people. BMJ Open 6(12):e015009. Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Maximus Berger
    • 1
    • 2
    • 3
    • 4
  • G. Paul Amminger
    • 3
    • 4
  • Robyn McDermott
    • 5
    • 6
  • Paul C. Guest
    • 7
  • Zoltán Sarnyai
    • 1
    • 2
    Email author
  1. 1.Laboratory of Psychiatric Neuroscience, Australian Institute of Tropical Health and MedicineJames Cook UniversityTownsvilleAustralia
  2. 2.Discipline of Biomedicine, College of Public Health, Medicine and Veterinary SciencesJames Cook UniversityTownsvilleAustralia
  3. 3.OrygenThe National Centre of Excellence in Youth Mental HealthParkvilleAustralia
  4. 4.Centre for Youth Mental HealthUniversity of MelbourneParkvilleAustralia
  5. 5.Centre for Chronic Disease Prevention, Australian Institute of Tropical Health and Medicine (AITHM), College of Public Health, Medical and Veterinary SciencesJames Cook UniversityCairnsAustralia
  6. 6.School of Health SciencesUniversity of South AustraliaAdelaideAustralia
  7. 7.Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of BiologyUniversity of Campinas (UNICAMP)CampinasBrazil

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