Whole-Slide Imaging of Esophageal Squamous Cell Carcinoma

  • Alfred K. LamEmail author
  • Melissa Leung
Part of the Methods in Molecular Biology book series (MIMB, volume 2129)


Whole-slide imaging (WSI) contributes to medical education, collaboration, quality assurance, examination, and consultation in pathology. The images obtained from WSI are of high quality and could be stored indefinitely. In research involving esophageal squamous cell carcinoma, the combination of WSI and image processing program allows effective interpretations of expressions of various immunomarkers related to pathogenesis, prognosis, and response to therapy in tissue microarray sections. The operation and basic principles of whole-slide imaging of esophageal squamous cell carcinoma are also presented. Common use of WSI will occur with modifications of the whole-slide imaging scanners to adapt to the workflows in diagnostic and research laboratories.

Key words

Whole-slide imaging Scanning Ki-67 Esophageal Squamous carcinoma Image analysis TMA 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Cancer Molecular Pathology, School of MedicineGriffith UniversityGold CoastAustralia
  2. 2.Cancer Molecular Pathology of School of MedicineGriffith UniversityGold CoastAustralia

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