Macroscopic Examination of Surgical Specimen of Esophageal Squamous Cell Carcinoma

  • Alfred K. LamEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2129)


Macroscopic examination of the surgical specimen of esophageal squamous cell carcinoma by pathologist is important for quality clinical management, research, as well as education purposes. The process includes dissection of the specimen, identification of the lesion, measurements, and taking appropriate samples for histopathological examination. The basic principle of the examination is to study the characteristics and extent of the cancer. In addition, examination of proximal resection margin and circumferential resection margin are important in the cancer. A standardized approach for macroscopic examination by professionals is needed for accurate diagnosis and to optimize the use of the surgical specimen with esophageal squamous cell carcinoma.

Key words

Esophageal squamous cell carcinoma Macroscopic Cut up Resection 


  1. 1.
    Dai W, Ko JMY, Choi SSA, Yu Z, Ning L, Zheng H, Gopalan V, Chan KT, Lee NP, Chan KW, Law SY, Lam AK, Lung ML (2017) Whole-exome sequencing reveals critical genes underlying metastasis in oesophageal squamous cell carcinoma. J Pathol 242:500–510CrossRefGoogle Scholar
  2. 2.
    Haque MH, Gopalan V, Chan KW, Shiddiky MJ, Smith RA, Lam AK (2016) Identification of novel FAM134B (JK1) mutations in oesophageal squamous cell carcinoma. Sci Rep 6:29173CrossRefGoogle Scholar
  3. 3.
    Wong ML, Tao Q, Fu L, Wong KY, Qiu GH, Law FB, Tin PC, Cheung WL, Lee PY, Tang JC, Tsao GS, Lam KY, Law S, Wong J, Srivastava G (2006) Aberrant promoter hypermethylation and silencing of the critical 3p21 tumour suppressor gene, RASSF1A, in Chinese oesophageal squamous cell carcinoma. Int J Oncol 28:767–773PubMedGoogle Scholar
  4. 4.
    Hu YC, Lam KY, Law S, Wong J, Srivastava G (2001) Profiling of differentially expressed cancer-related genes in esophageal squamous cell carcinoma (ESCC) using human cancer cDNA arrays: overexpression of oncogene MET correlates with tumor differentiation in ESCC. Clin Cancer Res 7:3519–3525PubMedGoogle Scholar
  5. 5.
    Hu YC, Lam KY, Law S, Wong J, Srivastava G (2001) Identification of differentially expressed genes in esophageal squamous cell carcinoma (ESCC) by cDNA expression array: overexpression of Fra-1, Neogenin, Id-1, and CDC25B genes in ESCC. Clin Cancer Res 7:2213–2221PubMedGoogle Scholar
  6. 6.
    Tang JC, Wan TS, Wong N, Pang E, Lam KY, Law SY, Chow LM, Ma ES, Chan LC, Wong J, Srivastava G (2001) Establishment and characterization of a new xenograft-derived human esophageal squamous cell carcinoma cell line SLMT-1 of Chinese origin. Cancer Genet Cytogenet 124:36–41CrossRefGoogle Scholar
  7. 7.
    Hu Y, Lam KY, Wan TS, Fang W, Ma ES, Chan LC, Srivastava G (2000) Establishment and characterization of HKESC-1, a new cancer cell line from human esophageal squamous cell carcinoma. Cancer Genet Cytogenet 118:112–120CrossRefGoogle Scholar
  8. 8.
    Hu YC, Lam KY, Law SY, Wan TS, Ma ES, Kwong YL, Chan LC, Wong J, Srivastava G (2002) Establishment, characterization, karyotyping, and comparative genomic hybridization analysis of HKESC-2 and HKESC-3: two newly established human esophageal squamous cell carcinoma cell lines. Cancer Genet Cytogenet 135:120–127CrossRefGoogle Scholar
  9. 9.
    Ip JC, Ko JM, Yu VZ, Chan KW, Lam AK, Law S, Tong DK, Lung ML (2015) A versatile orthotopic nude mouse model for study of esophageal squamous cell carcinoma. Biomed Res Int 2015:910715CrossRefGoogle Scholar
  10. 10.
    Gopalan V, Dissabandara L, Nirthanan S, Forwood MR, Lam AK (2016) Integrating gross pathology into teaching of undergraduate medical science students using human cadavers. Pathol Int 66:511–517CrossRefGoogle Scholar
  11. 11.
    Ariana A, Amin M, Pakneshan S, Dolan-Evans E, Lam AK (2016) Integration of traditional and e-learning methods to improve learning outcomes for dental students in histopathology. J Dent Educ 80:1140–1148PubMedGoogle Scholar
  12. 12.
    Gopalan V, Kasem K, Pillai S, Olveda D, Ariana A, Leung M, Lam AKY (2018) Evaluation of multidisciplinary strategies and traditional approaches in teaching pathology in medical students. Pathol Int 68:459–466CrossRefGoogle Scholar
  13. 13.
    Lam AK, Veitch J, Hays R (2005) Resuscitating the teaching of anatomical pathology in undergraduate medical education: web-based innovative clinicopathological cases. Pathology 37:360–363CrossRefGoogle Scholar
  14. 14.
    Kumarasinghe MP, Brown IS, Charlton A, De Boer B, Eckstein R, Epari K, Gill A. Lam A, Lauwers G, Raftopoulos S, Price T (2013) Tumours of the oesophagus and gastro-oesophageal junction structured reported protocol, 1st edition, Published on web by Royal College of Pathologists of Australasia.
  15. 15.
    Lam KY, Ma LT, Wong J (1996) Measurement of extent of spread of oesophageal squamous carcinoma by serial sectioning. J Clin Pathol 49:124–129CrossRefGoogle Scholar
  16. 16.
    Lam KY, Law SY, Loke SL, Fok M, Ma LT (1996) Double sarcomatoid carcinomas of the oesophagus. Pathol Res Pract 192(6):604–609CrossRefGoogle Scholar
  17. 17.
    Priante AV, Castilho EC, Kowalski LP (2011) Second primary tumors in patients with head and neck cancer. Curr Oncol Rep 13:132–137CrossRefGoogle Scholar
  18. 18.
    Carter JT (2015) Electronic medical records and quality improvement. Neurosurg Clin N Am 26:245–251CrossRefGoogle Scholar
  19. 19.
    Kim SW, Roh J, Park CS (2016) Immunohistochemistry for pathologists: protocols, pitfalls, and tips. J Pathol Transl Med 50:411–418CrossRefGoogle Scholar
  20. 20.
    Evers DL, Fowler CB, Cunningham BR, Mason JT, O’Leary TJ (2011) The effect of formaldehyde fixation on RNA: optimization of formaldehyde adduct removal. J Mol Diagn 13:282–288CrossRefGoogle Scholar
  21. 21.
    Hays R, Veitch J, Lam A (2005) Teaching clinical pathology by flexible delivery in rural sites. Aust J Rural Health 13:232–235CrossRefGoogle Scholar
  22. 22.
    Rampy BA, Glassy EF (2015) Pathology gross photography: the beginning of digital pathology. Surg Pathol Clin 8:195–211CrossRefGoogle Scholar
  23. 23.
    Yang YS, Wang YC, Deng HY, Yuan Y, Wang ZQ, He D, Chen LQ (2018) Prognostic value of circumferential resection margin in T3N0M0 esophageal squamous cell carcinoma. Ann Transl Med 6:303CrossRefGoogle Scholar
  24. 24.
    Williams AS, Hache KD (2014) Recognition and discrimination of tissue-marking dye color by surgical pathologists: recommendations to avoid errors in margin assessment. Am J Clin Pathol 142:355–361CrossRefGoogle Scholar
  25. 25.
    Tang JC, Lam KY, Law S, Wong J, Srivastava G (2001) Detection of genetic alterations in esophageal squamous cell carcinomas and adjacent normal epithelia by comparative DNA fingerprinting using inter-simple sequence repeat PCR. Clin Cancer Res 7:1539–1545PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Cancer Molecular Pathology of School of MedicineGriffith UniversityGold CoastAustralia

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