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Using iRFP Genetic Labeling Technology to Track Tumorogenesis of Transplanted CRISPR/Cas9-Edited iPSC in Skeletal Muscle

  • Yue Jin
  • Yan Shen
  • Neal L. Weintraub
  • Yaoliang TangEmail author
Protocol
  • 129 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2126)

Abstract

Tumorigenesis and attendant safety risks are significant concerns of induced pluripotent stem cell (iPSC)-based therapies. Thus, it is crucial to evaluate iPSC proliferation, differentiation, and tumor formation after transplantation. Several approaches have been employed for tracking the donor cells, including fluorescent protein and luciferase, but both have limitations. Here, we introduce a protocol using iRFP genetic labeling technology to track tumor formation of iPSCs in skeletal muscle after CRISPR/Cas9 gene editing.

Key words

Infrared fluorescent protein (iRFP) Induced pluripotent stem cells (iPSC) Tumor formation CRISPR/Cas9 

Notes

Acknowledgements

Funding: N. Weintraub and Y. Tang were partially supported by the American Heart Association: GRNT31430008, NIH-AR070029, NIH-HL086555, and NIH-HL134354.

Conflict of Interest: All authors declare that he/she has no conflict of interest.

This article does not contain any studies with human participants performed by any of the authors.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Yue Jin
    • 1
  • Yan Shen
    • 1
  • Neal L. Weintraub
    • 1
  • Yaoliang Tang
    • 1
    Email author
  1. 1.Vascular Biology Center, Medical College of GeorgiaAugusta UniversityAugustaUSA

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