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Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations

  • Tej B. ShresthaEmail author
  • Matthew T. Basel
Protocol
  • 117 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2126)

Abstract

Luminol-based bioluminescence imaging allows noninvasive tracking of oxidatively active cells such as neutrophils. Luminol is given intravenously or intraperitoneally, followed by bioluminescence imaging at 425 nm. Here we describe a method for tracking neutrophil extravasation into an inflammatory site, especially focusing on mammary carcinoma.

Key words

Myeloperoxidase NADPH oxidase Neutrophil Luminol Bioluminescence 

References

  1. 1.
    Kaaru E et al (2016) Molecular imaging in preclinical models of IBD with nuclear imaging techniques: state-of-the-art and perspectives. Inflamm Bowel Dis 22(10):2491–2498CrossRefGoogle Scholar
  2. 2.
    von der Haar K et al (2015) Lost signature: progress and failures in in vivo tracking of implanted stem cells. Appl Microbiol Biotechnol 99(23):9907–9922CrossRefGoogle Scholar
  3. 3.
    Hampton MB, Kettle AJ, Winterbourn CC (1998) Inside the neutrophil phagosome: oxidants, myeloperoxidase, and bacterial killing. Blood 92(9):3007–3017CrossRefGoogle Scholar
  4. 4.
    Treffers LW et al (2016) Neutrophils in cancer. Immunol Rev 273(1):312–328CrossRefGoogle Scholar
  5. 5.
    Klebanoff SJ (2005) Myeloperoxidase: friend and foe. J Leukoc Biol 77(5):598–625CrossRefGoogle Scholar
  6. 6.
    Chen WT, Tung CH, Weissleder R (2004) Imaging reactive oxygen species in arthritis. Mol Imaging 3(3):159–162CrossRefGoogle Scholar
  7. 7.
    Lundqvist H, Dahlgren C (1996) Isoluminol-enhanced chemiluminescence: a sensitive method to study the release of superoxide anion from human neutrophils. Free Radic Biol Med 20(6):785–792CrossRefGoogle Scholar
  8. 8.
    Gross S et al (2009) Bioluminescence imaging of myeloperoxidase activity in vivo. Nat Med 15(4):455–461CrossRefGoogle Scholar
  9. 9.
    Alshetaiwi HS et al (2013) Luminol-based bioluminescence imaging of mouse mammary tumors. J Photochem Photobiol B 127:223–228CrossRefGoogle Scholar
  10. 10.
    Davis RW et al (2019) Luminol Chemiluminescence reports photodynamic therapy-generated neutrophil activity in vivo and serves as a biomarker of therapeutic efficacy. Photochem Photobiol 95(1):430–438CrossRefGoogle Scholar
  11. 11.
    Breyne K et al (2017) Preconditioning with lipopolysaccharide or Lipoteichoic acid protects against Staphylococcus aureus mammary infection in mice. Front Immunol 8:833CrossRefGoogle Scholar
  12. 12.
    Veziant J et al (2016) Association of colorectal cancer with pathogenic Escherichia coli: focus on mechanisms using optical imaging. World J Clin Oncol 7(3):293–301CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Anatomy and Physiology, College of Veterinary MedicineKansas State UniversityManhattanUSA
  2. 2.Nanotechnology Innovation Center of Kansas StateKansas State UniversityManhattanUSA

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