Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations

  • Tej B. ShresthaEmail author
  • Matthew T. Basel
Part of the Methods in Molecular Biology book series (MIMB, volume 2126)


Luminol-based bioluminescence imaging allows noninvasive tracking of oxidatively active cells such as neutrophils. Luminol is given intravenously or intraperitoneally, followed by bioluminescence imaging at 425 nm. Here we describe a method for tracking neutrophil extravasation into an inflammatory site, especially focusing on mammary carcinoma.

Key words

Myeloperoxidase NADPH oxidase Neutrophil Luminol Bioluminescence 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Anatomy and Physiology, College of Veterinary MedicineKansas State UniversityManhattanUSA
  2. 2.Nanotechnology Innovation Center of Kansas StateKansas State UniversityManhattanUSA

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