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Cell Tracking pp 177-190 | Cite as

Comparison of the Efficacy and Sensitivity of Alternative PET Reporter Gene/PET Reporter Probe Systems That Minimize Biological Variables

  • Shili Xu
  • Harvey R. HerschmanEmail author
Protocol
  • 110 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2126)

Abstract

Positron emission tomography (PET) reporter genes (PRGs), when coupled with positron-emitting PET reporter probes (PRPs), are useful for tracking specific cell populations in cell-based therapies, in transgenic animal models, and in xenograft tumor progression experiments. The activities of incorporated PRGs in targeted cells can be monitored noninvasively by PET imaging in preclinical in vivo studies and clinical applications following systemic administration of the appropriate PRG. Here we describe a method that minimizes both design and variability of vector delivery vehicles for alternative PRGs and biological variability of the in vivo target when comparing the efficacy, sensitivity, and specificity of alternative PRG/PRP combinations for in vivo PRG imaging. The principles described for comparing alternative PRG/PRP reporter gene systems can be applied to comparisons of alternative fluorescence, bioluminescence, single-photon emission computerized tomography (SPECT), and magnetic resonance imaging (MRI) reporter genes.

Key words

Positron emission tomography PET reporter genes PET reporter probes Reporter gene imaging Adenovirus 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Molecular and Medical Pharmacology, David Geffen School of MedicineUniversity of California Los AngelesLos AngelesUSA
  2. 2.Crump Institute for Molecular Imaging, David Geffen School of MedicineUniversity of California Los AngelesLos AngelesUSA
  3. 3.Jonsson Comprehensive Cancer Center, David Geffen School of MedicineUniversity of California Los AngelesLos AngelesUSA
  4. 4.Department of Biological Chemistry, David Geffen School of MedicineUniversity of California Los AngelesLos AngelesUSA
  5. 5.Molecular Biology Institute, David Geffen School of MedicineUniversity of California Los AngelesLos AngelesUSA

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