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Cell Tracking pp 141-153 | Cite as

Stem Cell Tracking with Nanoparticle-Based Ultrasound Contrast Agents

  • Fang ChenEmail author
  • Jesse V. Jokerst
Protocol
  • 111 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2126)

Abstract

Cell therapy is revolutionizing modern medicine. To promote this emerging therapy, the ability to image and track therapeutic cells is critical to monitor the progress of the treatment. Ultrasound imaging is promising in tracking therapeutic cells but suffers from poor contrast against local tissues. Therefore, it is critical to increase the ultrasound contrast of therapeutic cells over local tissue at the injection site. Here, we describe a method to increase the ultrasound intensity of therapeutic cells with nanoparticles to make the injected therapeutic cells more visible.

Key words

Cell tracking Ultrasound imaging Contrast agents Nanoparticles Mesenchymal stem cells 

Notes

Acknowledgement

This work was supported by NIH (R00 HL117048 and DP2 HL137187) and the American Cancer Society Institutional Research (grant number 14-250-42). F. Chen also acknowledges Eric Zhao for proofreading the chapter.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Nanoengineering, Materials Science and Engineering ProgramUniversity of CaliforniaSan DiegoUSA
  2. 2.Department of OphthalmologyStanford UniversityStanfordUSA
  3. 3.Department of RadiologyUniversity of CaliforniaSan DiegoUSA

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