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Equine Influenza Culture Methods

  • Thomas M. ChambersEmail author
  • Stephanie E. Reedy
Protocol
  • 95 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2123)

Abstract

Equine influenza viruses are cultured in embryonated chicken eggs or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.

Key words

Embryonated eggs Mammalian influenza MDCK Plaque assay TCID assay 

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Copyright information

© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply. 2020

Authors and Affiliations

  1. 1.Maxwell H. Gluck Equine Research Center, Department of Veterinary ScienceUniversity of KentuckyLexingtonUSA

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