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Physico-chemical Properties of Virus in Crude Sap

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Protocol
  • 48 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The physico-chemical properties of the virus in crude sap such as dilution end point, thermal inactivation point and longevity in vitro would provide useful data on the concentration of the virus in the living host and its stability that would help in the transmission and virus purification experiments. Although, these properties are not being used at present to identify and characterize a plant virus, but still they are quite useful in getting idea about concentration and storage duration of a virus in active state in crude sap for future course of study.

Key words

Dilution end point Thermal inactivation point Longevity in vitro 

References

  1. Francki RIB (1980) Limited value of thermal inactivation point, longevity in vitro and dilution end point as criteria for the characterization, identification and classification of plant viruses. Intervirology 13:91–98CrossRefGoogle Scholar
  2. Hill SA (1984) Methods in plant virology. Blackwell, OxfordGoogle Scholar
  3. Kurstak E (1981) Hand book of plant virus infections. Comparative diagnosis. Elsevier, AmsterdamGoogle Scholar
  4. Smith KM (1972) A textbook of plant virus diseases. Longman, LondonGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

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