Recombinase Polymerase Amplification
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Recombinase polymerase amplification (RPA) assay is a single tube, amplification method that is carried out at a constant single temperature used for detection of DNA. It can also detect RNA by addition of reverse transcriptase enzyme to the RPA reaction without any additional step to produce cDNA. The amplification process is very quick and can be completed in 10–15 min. Because it is performed at a constant temperature, it requires just an equipment to maintain the set temperature like an incubator. In most cases, the optimum temperature for performing RPA lies between 37 and 42 °C. Thus RPA is a low-cost, rapid, point-of-care diagnostic assay. It is being successfully used for the detection of many plant viruses.
Key wordsIsothermal amplification Virus detection
- Lau HY, Botella JR (2017) Advanced DNA based point of care diagnostic methods for plant disease detection. Plant Sci 8:2016Google Scholar