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Recombinase Polymerase Amplification

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Protocol
  • 48 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Recombinase polymerase amplification (RPA) assay is a single tube, amplification method that is carried out at a constant single temperature used for detection of DNA. It can also detect RNA by addition of reverse transcriptase enzyme to the RPA reaction without any additional step to produce cDNA. The amplification process is very quick and can be completed in 10–15 min. Because it is performed at a constant temperature, it requires just an equipment to maintain the set temperature like an incubator. In most cases, the optimum temperature for performing RPA lies between 37 and 42 °C. Thus RPA is a low-cost, rapid, point-of-care diagnostic assay. It is being successfully used for the detection of many plant viruses.

Key words

Isothermal amplification Virus detection 

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

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