Advertisement

Loop-Mediated Isothermal Amplification (LAMP)

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Protocol
  • 50 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Loop-mediated isothermal amplification (LAMP) can amplify nucleic acid with high specificity, sensitivity and speed under isothermal conditions. The LAMP method uses four to six primers and a DNA polymerase with strand-displacing activity to generate amplification products. The products can be detected by agarose gel electrophoresis or visually by turbidity or colour changes. The amplified products of LAMP are also visible as ladder-like patterns on a gel. LAMP methods have been used successfully for detecting both the RNA and DNA plant viruses.

Key words

Reverse transcription (RT)-LAMP Virus detection Real-time LAMP Turbidity, isothermal reaction 

References

  1. Abdullahi UF, Rochman N, Wan R, Wan T, Ahmadu S, Anas M, Aliyu S, Baig A (2015) Loop-mediated isothermal amplification (LAMP), an innovation in gene amplification: bridging the gap in molecular diagnostics: a review. Indian J Sci and Tech 8:1–12CrossRefGoogle Scholar
  2. Bhat AI, Siljo A, Deeshma KP (2013) Rapid detection of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) by loop-mediated isothermal amplification (LAMP). J Virol Methods 193:190–196CrossRefGoogle Scholar
  3. Fukuta S, Ohishi K, Yoshida K, Mizukami Y, Ishida A, Kanbe M (2004) Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of Tomato spotted wilt virus from chrysanthemum. J Virol Methods 121:49–55CrossRefGoogle Scholar
  4. Keizerweerda AT, Chandra A, Grisham MP (2015) Development of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of Sugarcane mosaic virus and Sorghum mosaic virus in sugarcane. J Virol Methods 212:23–29CrossRefGoogle Scholar
  5. Mori Y, Nagamine K, Tomita N, Notomi T (2001) Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289:150–154CrossRefGoogle Scholar
  6. Nagamine K, Hase T, Notomi T (2002) Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16:223–229CrossRefGoogle Scholar
  7. Nie X (2005) Reverse transcription loop-mediated isothermal amplification of DNA for detection of Potato virus Y. Plant Dis 89:605–610CrossRefGoogle Scholar
  8. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:e63CrossRefGoogle Scholar
  9. Siljo A, Bhat AI (2014) Reverse transcription loop-mediated isothermal amplification assay for rapid and sensitive detection of Banana bract mosaic virus in cardamom (Elettaria cardamomum). Eur J Plant Pathol 138:209–214CrossRefGoogle Scholar
  10. Tomita N, Mori Y, Kanda H, Notomi T (2008) Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc 3:877–882CrossRefGoogle Scholar
  11. Zhang X, Lowe SB, Gooding JJ (2014) Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP). Biosens Bioelectron 61:491–499CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

Personalised recommendations