Loop-Mediated Isothermal Amplification (LAMP)
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Loop-mediated isothermal amplification (LAMP) can amplify nucleic acid with high specificity, sensitivity and speed under isothermal conditions. The LAMP method uses four to six primers and a DNA polymerase with strand-displacing activity to generate amplification products. The products can be detected by agarose gel electrophoresis or visually by turbidity or colour changes. The amplified products of LAMP are also visible as ladder-like patterns on a gel. LAMP methods have been used successfully for detecting both the RNA and DNA plant viruses.