Loop-Mediated Isothermal Amplification (LAMP)

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Part of the Springer Protocols Handbooks book series (SPH)


Loop-mediated isothermal amplification (LAMP) can amplify nucleic acid with high specificity, sensitivity and speed under isothermal conditions. The LAMP method uses four to six primers and a DNA polymerase with strand-displacing activity to generate amplification products. The products can be detected by agarose gel electrophoresis or visually by turbidity or colour changes. The amplified products of LAMP are also visible as ladder-like patterns on a gel. LAMP methods have been used successfully for detecting both the RNA and DNA plant viruses.

Key words

Reverse transcription (RT)-LAMP Virus detection Real-time LAMP Turbidity, isothermal reaction 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

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