Advertisement

Determination of Coat Protein Molecular Weight of Viruses

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Protocol
  • 47 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Molecular weight (mw) and number of polypeptides in the coat protein is one of the criteria used for the identification of virus species. The determination of molecular weight of the viral coat protein involves denaturing of protein subunits in the presence of sodium dodecyl sulphate (SDS) by heating in boiling water. The denatured protein is further run on an SDS-poly acrylamide gel electrophoresis (SDS-PAGE) along with protein markers of known molecular weight. The molecular weight of the unknown virus protein is estimated by comparing its mobility in the gel, with that of other proteins of known molecular weight.

Key words

SDS-PAGE Resolving gel Staking gel Vertical gel electrophoresis Purified virus 

References

  1. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefGoogle Scholar
  2. Sambrook J, Russel DW (2001) Molecular cloning, vol I-III, 3rd edn. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

Personalised recommendations