Determination of Coat Protein Molecular Weight of Viruses

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Part of the Springer Protocols Handbooks book series (SPH)


Molecular weight (mw) and number of polypeptides in the coat protein is one of the criteria used for the identification of virus species. The determination of molecular weight of the viral coat protein involves denaturing of protein subunits in the presence of sodium dodecyl sulphate (SDS) by heating in boiling water. The denatured protein is further run on an SDS-poly acrylamide gel electrophoresis (SDS-PAGE) along with protein markers of known molecular weight. The molecular weight of the unknown virus protein is estimated by comparing its mobility in the gel, with that of other proteins of known molecular weight.

Key words

SDS-PAGE Resolving gel Staking gel Vertical gel electrophoresis Purified virus 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

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