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Formulation and Characterization of Antithrombin Perfluorocarbon Nanoparticles

  • Alexander J. Wilson
  • Qingyu Zhou
  • Ian Vargas
  • Rohun Palekar
  • Ryan Grabau
  • Hua Pan
  • Samuel A. WicklineEmail author
Protocol
  • 146 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2118)

Abstract

Thrombin, a major protein involved in the clotting cascade by the conversion of inactive fibrinogen to fibrin, plays a crucial role in the development of thrombosis. Antithrombin nanoparticles enable site-specific anticoagulation without increasing bleeding risk. Here we outline the process of making and the characterization of bivalirudin and d-phenylalanyl-l-prolyl-l-arginyl-chloromethyl ketone (PPACK) nanoparticles. Additionally, the characterization of these nanoparticles, including particle size, zeta potential, and quantification of PPACK/bivalirudin loading, is also described.

Key words

Perfluorocarbon nanoparticles Thrombin PPACK Bivalirudin Anticoagulation 

Notes

Acknowledgments

The manuscript was edited by Enrico Ferrari and Mikhail Soloviev.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alexander J. Wilson
    • 1
  • Qingyu Zhou
    • 2
  • Ian Vargas
    • 1
  • Rohun Palekar
    • 3
  • Ryan Grabau
    • 1
  • Hua Pan
    • 1
  • Samuel A. Wickline
    • 1
    Email author
  1. 1.The USF Health Heart InstituteUniversity of South FloridaTampaUSA
  2. 2.College of PharmacyUniversity of South FloridaTampaUSA
  3. 3.Department of Biomedical EngineeringWashington University in St. LouisSt. LouisUSA

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