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Preparation of Hyaluronic Acid-Based Nanoparticles for Macrophage-Targeted MicroRNA Delivery and Transfection

  • Neha N. ParayathEmail author
  • Mansoor M. Amiji
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Part of the Methods in Molecular Biology book series (MIMB, volume 2118)

Abstract

Skewing the macrophage polarity to achieve a favorable phenotype is a recently investigated therapeutic strategy in multiple disease/dysfunctional conditions such as inflammation, tumors, autoimmune disorders, and tissue repairs. However, delivering the therapeutic agent specifically to the macrophages has been a challenge in this field. Here, we describe the synthesis of hyaluronic acid (HA)-based nanoparticles for targeting CD44 receptors on the macrophages. The HA backbone is modified with cationic polyethyleneimine (PEI) for efficient encapsulation of microRNA into the self-assembling nanoparticles for targeted delivery to macrophages.

Key words

Hyaluronic acid nanoparticles CD44 Macrophages Poly(ethyleneimine) Targeted delivery M1 and M2 polarization MicroRNA-125b 

Notes

Acknowledgments

The manuscript was edited by Enrico Ferrari and Mikhail Soloviev. Financial support for this work was provided by the United States National Cancer Institute of the National Institute of Health through grants R21-CA179652 and R56-CA198492, and the Northeastern University-Dana Farber Cancer Center Joint Program on Cancer Drug Development.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Pharmaceutical Sciences, School of PharmacyNortheastern UniversityBostonUSA
  2. 2.Clinical Research DivisionFred Hutchinson Cancer Research CenterSeattleUSA

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