Preparation of Lipid–Peptide–DNA (LPD) Nanoparticles and Their Use for Gene Transfection
- 162 Downloads
Therapeutic gene delivery systems offer the potential for the treatment of a range of inherited and acquired inherited diseases. In contrast with viral gene vectors, the nonviral gene vectors provide a safer alternative and additional advantages such as the improved delivery efficiency, low cost, and often unlimited capacity to package DNA. Here we describe the preparation of a nonviral gene delivery technique based on lipid–peptide–DNA (LPD) complexes. The size of LPD particles is in the nanometer range. The use of these nanoparticulate LPDs results in high efficiency transfections and a high level of gene expression in vitro. LPDs provide a convenient and efficient tool for gene delivery in gene therapy.
Key wordsLiposome Nonviral gene vector Gene transfection Flow cytometry
The manuscript was edited by Mikhail Soloviev.