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Preparation of Lipid–Peptide–DNA (LPD) Nanoparticles and Their Use for Gene Transfection

  • Fan Zhang
  • Hao-Ying LiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2118)

Abstract

Therapeutic gene delivery systems offer the potential for the treatment of a range of inherited and acquired inherited diseases. In contrast with viral gene vectors, the nonviral gene vectors provide a safer alternative and additional advantages such as the improved delivery efficiency, low cost, and often unlimited capacity to package DNA. Here we describe the preparation of a nonviral gene delivery technique based on lipid–peptide–DNA (LPD) complexes. The size of LPD particles is in the nanometer range. The use of these nanoparticulate LPDs results in high efficiency transfections and a high level of gene expression in vitro. LPDs provide a convenient and efficient tool for gene delivery in gene therapy.

Key words

Liposome Nonviral gene vector Gene transfection Flow cytometry 

Notes

Acknowledgments

The manuscript was edited by Mikhail Soloviev.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Medical Research CouncilLaboratory of Molecular BiologyCambridgeUK
  2. 2.Biomanufacturing Research Centre, School of Mechanical and Electronic EngineeringSoochow UniversitySuzhouChina

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