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Antimicrobial Applications of Silver Nanoparticles to E. coli Colony Biofilms

  • James P. McEvoyEmail author
  • Kayra Genc
  • Priya Loi
  • William J. Walker
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 2118)

Abstract

Bacterial biofilms can cause problems in various arenas, from the fouling of water processing equipment to persistent in vivo infections. Silver nanoparticles are promising antimicrobial agents with activity against biofilm bacteria. Here we describe the synthesis of antimicrobial silver nanoparticles and the measurement of their antimicrobial activity against E. coli colony biofilms, which is a popular in vitro biofilm model for antibiotic assays.

Key words

Silver nanoparticles Silver nitrate Sodium borohydride Polyvinylpyrrolidone Aqueous synthesis E. coli Antimicrobial Antibacterial Biofilms Antibiotics biocides Resistance Scanning electron microscopy 

Notes

Acknowledgments

The manuscript was edited by Enrico Ferrari and Mikhail Soloviev. The authors thank Sharon Gibbons for her help with the scanning electron microscopy.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • James P. McEvoy
    • 1
    Email author
  • Kayra Genc
    • 1
  • Priya Loi
    • 1
  • William J. Walker
    • 1
  1. 1.Centre for Biomedical Sciences, School of Biological Sciences, Royal HollowayUniversity of LondonSurreyUK

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