In Vitro Labeling Mesenchymal Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Efficacy and Cytotoxicity
- 157 Downloads
Mesenchymal stem cell (MSC) therapy has emerged as a potential therapeutic option for several diseases due to their unique properties of releasing important bioactive factors. Despite the advances in stem cell therapy, it is still difficult to accurately determine the mechanisms of cell activities after in vivo transplantation. The application of noninvasive cell tracking approaches is important to determine tissue distribution and the lifetime of stem cells following their injection, which consequently provides knowledge about the mechanisms of stem cell tissue repair. Superparamagnetic iron oxide nanoparticles (SPION) can provide a very useful tool for labeling and tracking stem cells by magnetic resonance imaging without causing toxic cellular effects and do not elicit any other side effects. Here we describe how to use SPIONs to label mesenchymal stem cells and evaluate efficacy and potential cytotoxicity in vitro.
Key wordsSuperparamagnetic iron oxide nanoparticles SPION Mesenchymal stem cells MSC Cellular labeling Labeling efficacy Cytotoxicity
The manuscript was edited by Enrico Ferrari and Mikhail Soloviev. The research was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq. The author thanks student Pietro Colonna for the Prussian Blue staining pictures.
- 6.Jasmin, Torres AL, Nunes HM et al (2011) Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging. J Nanobiotechnology 9:4. https://doi.org/10.1186/1477-3155-9-4CrossRefPubMedPubMedCentralGoogle Scholar
- 15.Bourrinet P, Bengele HH, Bonnemain B et al (2006) Preclinical safety and pharmacokinetic profile of ferumoxtran-10, an ultrasmall superparamagnetic iron oxide magnetic resonance contrast agent. Investig Radiol 41(3):313–324. https://doi.org/10.1097/01.rli.0000197669.80475.ddCrossRefGoogle Scholar