Collection, Transportation, and Preservation of Biological Evidences for DNA Analysis
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Application of DNA technology has revolutionized the field of forensic science. To obtain an appropriate result from any biological evidence, proper collection, transportation, and preservation/storage strategies should be employed for respective samples without manipulating the chain of custody (Fig. 3.1). Many factors contribute to the generation of a good DNA profile from a biological sample, i.e., the sensitivity of a PCR setup, rate and extent of degradation, purity, and DNA content of the sample. No biological evidence is resistant to degradation. Thus, careful collection and proper storage of the biological samples can provide a useful information when subjected to DNA typing analysis. Microbial or environmental degradation does not alter the genetic profile of a subject, but can impact adversely by obtaining a partial DNA profile from the sample. Evidences suitable for DNA examination are limited to biological samples; hence, a proper precaution for collection, transportation, and storage is required for these samples which has been discussed in details in this chapter.