Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus

  • Zhuang Ye
  • Ling Zhao
  • Qi Gao
  • Yanfang Jiang
  • Zhenyu Jiang
  • Cong-Qiu ChuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2108)


Interleukin (IL)-22 belongs to the IL-10 family of cytokines. IL-22 exerts its biological effects via members of the cytokine receptor family class 2. CD4+ T helper (Th) cells predominantly producing IL-22 have been designated as Th22 cells. IL-22/Th22 cells are functionally related to IL-17/Th17 cells, but are distinctly different. Both IL-22 and IL-17 are cytokines recruiting neutrophils in response to microbe invasion. In chronic inflammation, IL-22 mediates protective and regenerative processes, whereas IL-17 cytokines tend to induce inflammation. Studies found that increased IL-22 levels and Th22 cells in peripheral blood were associated with disease activity in patients with systemic lupus erythematosus (SLE), but decreased IL-22 and Th22 cells were also reported. Here we describe analysis of IL-22 and Th22 cells in peripheral blood quantified by flow cytometry, and correlate our findings with SLE disease activity.

Key words

Interleukin-22 Th22 cells Systemic lupus erythematosus ELISA Flow cytometry 



This work was supported by grants from the National Natural Science Foundation of China (No. 81501343), Bethune Plan Project of Jilin University (2015410). CQC was supported by Rheumatology Research Foundation Innovative and Pilot grants.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Zhuang Ye
    • 1
  • Ling Zhao
    • 1
  • Qi Gao
    • 1
  • Yanfang Jiang
    • 2
  • Zhenyu Jiang
    • 1
  • Cong-Qiu Chu
    • 3
    • 4
    Email author
  1. 1.Department of Rheumatology and Immunology, The First Bethune HospitalJilin UniversityChangchunChina
  2. 2.Department of Central Laboratory, The First Bethune HospitalJilin UniversityChangchunChina
  3. 3.Division of Arthritis and Rheumatic DiseasesOregon Health & Science UniversityPortlandUSA
  4. 4.Section of RheumatologyVA Portland Health Care SystemPortlandUSA

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