Interleukin-8 Induces Proliferation of Ovarian Cancer Cells in 3D Spheroids

  • Mohammad M. Uddin
  • Bijaya Gaire
  • Ivana VancurovaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2108)


Ovarian cancer (OC) is the most common cause of cancer deaths among gynecological malignancies. OC ascites contain multicellular spheroid aggregates, which exhibit increased pro-survival signaling, invasive behavior, and chemotherapeutic resistance. OC cells are characterized by an increased expression of the pro-inflammatory and pro-angiogenic chemokine interleukin-8 (IL-8, CXCL8), which increases their survival and migration, thus contributing to OC metastasis and angiogenesis. While previous studies have shown that IL-8 increases proliferation of OC cells grown in monolayer cultures, the effect of IL-8 on proliferation of OC cells grown in 3D spheroids has not been investigated. The spheroid 3D culture assays have been particularly useful in translational research since they allow cell-to-cell interactions that resemble tumor growth in vivo, while allowing easy cell manipulations and visualization. Here, we used the 3D spheroid culture assay to investigate the effect of IL-8 on OC cell proliferation. Using this assay, our results show that IL-8 significantly increases proliferation of OC cells grown in 3D spheroids.

Key words

Cancer cell proliferation CXCL8 3D culture Interleukin-8 Ovarian cancer Spheroid formation 



This work was supported by NIH grant CA202775 to I. Vancurova.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Mohammad M. Uddin
    • 1
  • Bijaya Gaire
    • 1
  • Ivana Vancurova
    • 1
    Email author
  1. 1.Department of Biological SciencesSt. John’s UniversityQueensUSA

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