Synthesis of PNA-Peptide Conjugates as Functional SNARE Protein Mimetics

  • Barbara E. Hubrich
  • Patrick M. Menzel
  • Benedikt Kugler
  • Ulf DiederichsenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2105)


PNA-peptide conjugates are versatile tools in chemical biology, which are employed in a variety of applications. Here, we present the synthesis of PNA-peptide conjugates that serve as SNARE protein-mimicking biooligomers. They resemble the structure of native SNARE proteins but exhibit a much simpler architecture. Incorporated into liposomes, they induce lipid mixing, so that they can be used to study the SNARE-mediated membrane fusion in a simplified setting in vitro. They consist of artificial SNARE recognition units made out of PNA oligomers, which are attached to the native linker and transmembrane domains of two neuronal SNAREs. The PNA-peptide conjugates are synthesized via solid-phase peptide synthesis in a continuous fashion starting with the peptide part, followed by assembly of the PNA recognition unit. On top, we describe a strategy to synthesize PNA-peptide conjugates in a fully automated fashion by using a peptide synthesizer.

Key words

Peptide nucleic acids PNA-peptide conjugates Membrane fusion SNARE protein mimetics Solid-phase peptide synthesis 



We gratefully acknowledge generous support from the Deutsche Forschungsgemeinschaft in the context of the SFB 803 (project B05).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Barbara E. Hubrich
    • 1
  • Patrick M. Menzel
    • 1
  • Benedikt Kugler
    • 1
  • Ulf Diederichsen
    • 1
    Email author
  1. 1.Institute for Organic and Biomolecular ChemistryUniversity of GöttingenGöttingenGermany

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