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RNA Chaperones pp 271-282 | Cite as

Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding

  • Heesoo Uhm
  • Sungchul HohngEmail author
Protocol
  • 303 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2106)

Abstract

Cotranscriptional RNA folding plays important roles in gene regulation steps such as splicing, transcription termination, and translation initiation. Progression of our understanding of cotranscriptional RNA folding mechanisms is still retarded by the lacking of experimental tools to study the kinetics of cotranscriptional RNA folding properly. In this chapter, we describe fluorescence resonance energy transfer (FRET) assay that enables the study of RNA cotranscriptional folding at the single-molecule level.

Key words

Cotranscriptional folding Single-molecule FRET Elongation complex reconstitution Riboswitch 

Notes

Acknowledgments

This work was supported by a Creative Research Initiative program (2009-0081562) to SH.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Physics and AstronomySeoul National UniversitySeoulRepublic of Korea
  2. 2.Institute of Applied PhysicsSeoul National UniversitySeoulRepublic of Korea

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