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Two-Dimensional Difference Gel Electrophoresis: A Gel-Based Proteomic Approach for Protein Analysis

  • Weimin GaoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2102)

Abstract

Two-dimensional difference gel electrophoresis (2D-DIGE) remains to be one of the most popular and versatile methods of protein separation among many proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE. Different from 2D-PAGE, proteins are pre-labeled with different fluorescent dyes, and different protein samples are run in one gel by this method. Therefore, 2D-DIGE not only carries the advantages of 2D-PAGE but also eliminates gel-to-gel variation and achieves high resolution, sensitivity, and reproducibility.

Key words

Two-dimensional difference gel electrophoresis Two-dimensional polyacrylamide gel electrophoresis Protein separation Proteomics 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Occupational and Environmental Health Sciences, School of Public HealthWest Virginia UniversityMorgantownUSA

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