The Gene Cluster Instability (GCI) Assay for Recombination
- 178 Downloads
A newly developed method for quantitatively detecting genomic restructuring in cultured human cell lines as the result of recombination is presented: the “gene cluster instability” (GCI) assay. The assay is physiological in that it detects spontaneous restructuring without the need for exogenous recombination-initiating treatments such as DNA damage. As an assay for genotoxicity, the GCI assay is complementary to well-established sister chromatid exchange (SCE) methods. Analysis of the U-2 OS osteosarcoma cell line is presented as an illustration of the method.
Key wordsGenotoxicity Recombination Gene cluster Genomic instability
- 11.Birren B, Lai E (1993) Pulsed field gel electrophoresis -- a practical guide. Academic Press, San Diego, CAGoogle Scholar