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The Gene Cluster Instability (GCI) Assay for Recombination

  • Michael W. Killen
  • Dawn M. Stults
  • Paola Marco-Casanova
  • Andrew J. PierceEmail author
Protocol
  • 178 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2102)

Abstract

A newly developed method for quantitatively detecting genomic restructuring in cultured human cell lines as the result of recombination is presented: the “gene cluster instability” (GCI) assay. The assay is physiological in that it detects spontaneous restructuring without the need for exogenous recombination-initiating treatments such as DNA damage. As an assay for genotoxicity, the GCI assay is complementary to well-established sister chromatid exchange (SCE) methods. Analysis of the U-2 OS osteosarcoma cell line is presented as an illustration of the method.

Key words

Genotoxicity Recombination Gene cluster Genomic instability 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Michael W. Killen
    • 1
    • 2
  • Dawn M. Stults
    • 3
    • 4
  • Paola Marco-Casanova
    • 5
  • Andrew J. Pierce
    • 6
    • 7
    Email author
  1. 1.Department of Microbiology, Immunology and Molecular GeneticsUniversity of KentuckyLexingtonUSA
  2. 2.Western Kentucky UniversityBowling GreenUSA
  3. 3.Graduate Center for ToxicologyUniversity of KentuckyLexingtonUSA
  4. 4.Clovis OncologyBoulderUSA
  5. 5.AstraZeneca IMED Biotech UnitCambridgeUK
  6. 6.AstraZeneca IMED Biotech UnitCambridgeUK
  7. 7.Department of Microbiology, Immunology and Molecular Genetics, Markey Cancer CenterUniversity of KentuckyLexingtonUSA

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