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Immobilization of Enzymes as Cross-Linked Enzyme Aggregates: General Strategy to Obtain Robust Biocatalysts

  • Susana Velasco-LozanoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2100)

Abstract

Among carrier-free immobilization techniques, cross-linked enzyme aggregates (CLEA) have been extensively described for a great number of diverse enzymes. During the last two decades, numerous efforts have been devoted to identify and understand the main variables involved in CLEA’s preparation process leading to robust immobilized biocatalysts. Since every enzyme immobilized as CLEA requires specific conditions and protocols, herein we provide a general preparation strategy where main parameters are highlighted and correlated with a possible desired improved enzyme feature.

Key words

CLEA Immobilization Enzyme Carrier-free Stabilization 

Notes

Acknowledgments

S. Velasco acknowledges the Mexican Council of Science and Technology (CONACyT) for the received postdoctoral fellowship, as well as the scientific illustrator Daniela Velasco (https://danielavelasco.myportfolio.com/scientific-illustration), for illustrating the Fig. 1.

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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Catálisis Heterogénea en Síntesis Orgánicas Selectivas (ISQCH-CSIC), Universidad de ZaragozaZaragozaSpain

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