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MAIT Cells pp 299-305 | Cite as

Magnetic Bead-Based Enrichment of Murine MAIT Cells

  • Samantha J. Winter
  • Andreas KruegerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2098)

Abstract

Mucosal-associated invariant T cells (MAIT) are abundant in humans, comprising up to 40% of liver T cells and 10% of peripheral blood T cells. However, understanding MAIT cell biology is hampered by the fact that they are fundamentally rare in standard laboratory mouse strains, such as C57BL/6. The discovery of MAIT cell ligands and recent advances in MR1-tetramer technology has provided a means for detecting murine MAIT cells, but low frequencies still hinder precise characterization. Here we describe how to accurately isolate rare MAIT cells from murine lymphocyte populations using MR1-tetramer technology combined with magnetic bead enrichment. Isolated MAIT cells can be used for downstream characterization or functional analysis.

Key words

MAIT cells Tetramers T cells Magnetic bead enrichment Flow cytometry 

Notes

Acknowledgments

MR1-5-OP-RU tetramer technology was developed jointly by Dr. James McCluskey, Dr. Jamie Rossjohn, and Dr. David Fairlie, and the material was produced by the NIH Tetramer Core Facility as permitted to be distributed by the University of Melbourne. This work was funded by grants from the German Research foundation (DFG) KR2320/5-1 and SFB902-B15 (to A.K.).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Institute for Molecular Medicine, Goethe University FrankfurtFrankfurt am MainGermany

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