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MAIT Cells pp 283-297 | Cite as

MAIT Cells in Type 1 Diabetes Mouse Models

  • Isabelle Nel
  • Lucie Beaudoin
  • Agnès LehuenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2098)

Abstract

MAIT cells are unconventional T cells expressing a semi-invariant αβ TCR, and they recognize bacterial metabolites via the highly conserved MR1 protein. MAIT cells interact with gut microbiota and literature reports alterations of gut homeostasis in type 1 diabetes (T1D), suggesting the involvement of MAIT cells in T1D. Since NOD mice is a well-established mouse model of T1D, MAIT cells were studied in these mice to evaluate their potential involvement in disease development. This chapter describes the material and methods required to characterize MAIT cells and to determine their function in T1D mouse models.

Key words

MAIT MR1 T1D NOD Intestinal microbiota Migration Cytokine Activation 

Notes

Acknowledgments

We thank M. Salou for help in analysis of in vitro MAIT cell activation (5-OP-RU/fecal supernatant dilution); the mouse and Cybio facilities of the Cochin Institute. This work was supported by grants from INSERM, CNRS, Laboratoire d’Excellence consortium Inflamex ANR-11-IDEX-0005-02, and the Fondation pour la Recherche Médicale (FRM grant number DEQ20140329520, EQU201903007779 to A.L.), Fondation Francophone pour la Recherche sur le Diabète to A.L., EFSD/JDRF/Lilly to A.L., Aide aux Jeunes Diabétiques fellowship to I.N., Agence National de la Recherche (ANR-17-CE14-0002-01 Diab1MAIT to A.L.).

Author Contributions: I.N. and L.B. wrote the review and A.L. supervised the writing of the review.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Isabelle Nel
    • 1
    • 2
    • 3
  • Lucie Beaudoin
    • 1
    • 2
    • 3
  • Agnès Lehuen
    • 1
    • 2
    • 3
    Email author
  1. 1.INSERM U1016, Institut CochinParisFrance
  2. 2.CNRS, UMR8104ParisFrance
  3. 3.Université Paris Descartes, Laboratoire d’Excellence Inflamex, Sorbonne Paris CitéParisFrance

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