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MAIT Cells pp 149-165 | Cite as

Quantification of Human MAIT Cell-Mediated Cellular Cytotoxicity and Antimicrobial Activity

  • Wan Rong Sia
  • Caroline Boulouis
  • Muhammad Yaaseen Gulam
  • Andrea Lay Hoon Kwa
  • Johan K. Sandberg
  • Edwin LeeansyahEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2098)

Abstract

The mucosa-associated invariant T (MAIT) cells represent the most abundant population of antimicrobial T cells in humans. When encountering cells infected with riboflavin-producing bacteria, this innate-like T cell population rapidly release a plethora of pro-inflammatory cytokines, mediates antimicrobial activity, and kill infected cells. Here, we describe methodological approaches and protocols to measure their cytotoxicity and antimicrobial effector function using multi-color flow cytometry-based and standard microbiological techniques. We provide specific guidance on protocols and describe potential pitfalls for each of the presented methodologies. Finally, we discuss potential applications and current limitations of our approaches to the study of human MAIT cell antimicrobial properties.

Key words

Human Mucosa-associated invariant T cells Cytolytic molecules Multi-color flow cytometry Antimicrobial 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Wan Rong Sia
    • 1
  • Caroline Boulouis
    • 2
  • Muhammad Yaaseen Gulam
    • 1
  • Andrea Lay Hoon Kwa
    • 1
    • 3
  • Johan K. Sandberg
    • 2
  • Edwin Leeansyah
    • 1
    • 2
    Email author
  1. 1.Program in Emerging Infectious DiseasesDuke-National University of Singapore Medical SchoolSingaporeSingapore
  2. 2.Department of Medicine, Center for Infectious MedicineKarolinska InstitutetStockholmSweden
  3. 3.Department of PharmacySingapore General HospitalSingaporeSingapore

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