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The Propagation and Quantification of Two Emerging Oncolytic Viruses: Vesicular Stomatitis (VSV) and Zika (ZIKV)

  • Robert E. Means
  • Sounak Ghosh Roy
  • Samuel G. KatzEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2097)

Abstract

Developments in genetic engineering have allowed researchers and clinicians to begin harnessing viruses to target and kill cancer cells, either through direct lysis or through recruitment of antiviral immune responses. Two powerful viruses in the fight against cancer are the single-stranded RNA viruses vesicular stomatitis virus and Zika virus. Here, we describe methods to propagate and titer these two viruses. We also describe a simple cell-killing assay to begin testing modified viruses for increased potential killing of glioblastoma cells.

Keywords

Oncolytic virus therapy Vesicular stomatitis virus Zika virus Glioblastoma Immunotherapeutics Viral titration Plaque assay Cell killing 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Robert E. Means
    • 1
  • Sounak Ghosh Roy
    • 1
  • Samuel G. Katz
    • 1
    Email author
  1. 1.Department of PathologyYale School of MedicineNew HavenUSA

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