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Measurement of Arabidopsis thaliana Nuclear Size and Shape

  • Kalyanikrishna
  • Pawel Mikulski
  • Daniel SchubertEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2093)

Abstract

Gene expression is tightly linked to the position of genes in the nucleus. Genomic regions associated with the nuclear envelope are usually repressed, including the heterochromatin carrying chromocenters. The shape and size of nuclei varies within tissues in plants and is dependent on proteins associated with the nuclear envelope. Here, we describe a protocol to isolate Arabidopsis thaliana nuclei and measure their size and morphology. Using this method, novel components regulating the nuclear envelope and chromatin association can be identified and analyzed.

Key words

Nuclear size Nuclear shape Nuclear envelope Chromatin 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Kalyanikrishna
    • 1
  • Pawel Mikulski
    • 1
    • 2
  • Daniel Schubert
    • 1
    Email author
  1. 1.Institute of Biology, Freie Universität BerlinBerlinGermany
  2. 2.John Innes CentreNorwichUK

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