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Chromatin Analysis of Metabolic Gene Clusters in Plants

  • Ancheng C. HuangEmail author
  • Hans-Wilhelm NützmannEmail author
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 2093)

Abstract

Plant metabolic gene clusters consist of neighboring genes that are involved in the biosynthesis of secondary or specialized metabolites. The genes within clusters are typically co-regulated, share a common set of chromatin marks, and code for the biosynthesis enzymes of a single metabolic pathway. Here, we describe three essential protocols for the basic analysis of metabolic gene clusters at transcription, histone modification, and metabolite level. The protocols are specified to clusters in the Arabidopsis thaliana genome and are transferable to other plant species.

Key words

Gene cluster Metabolism Chromatin modifications Triterpene 

Notes

Acknowledgments

This work was supported by startup funding from the Southern University of Science and Technology and Shenzhen municipal government (ACH) and the Royal Society University Research Fellowship (UF160138) (HWN).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Institute of Plant and Food Science, Department of BiologySouthern University of Science and TechnologyShenzhenChina
  2. 2.The Milner Centre for EvolutionUniversity of BathBathUK

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