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Multielectrode Array Recording of Mouse Retinas Transplanted with Stem Cell-Derived Retinal Sheets

  • Hung-Ya TuEmail author
  • Take Matsuyama
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2092)

Abstract

Retinal multielectrode array (MEA) recording allows us to examine the action potentials of retinal ganglion cells and field potentials of photoreceptors and bipolar cells. In addition to studying the retinal circuitry, it has become one of the standard examination tools for the characterization of stem cell-derived retinal transplantation in degenerated retinas. Besides the detection of responses to simple light stimulation, it is also necessary to consider the spatial correlation of the graft and the electrodes, in order to unbiasedly reveal the locally reconstructed retinal circuitry after transplantation. Here, we introduce our newly developed protocol of MEA recording and analysis that may serve as a standard for evaluating transplanted retinas.

Key words

Multielectrode array (MEA) Micro-electroretinogram (mERG) Spike sorting iPS/ES cell-derived 3D retina Retinal transplantation Metabotropic glutamate receptor type 6 (mGluR6) L-AP4 ON bipolar cells Retinal ganglion cells (RGCs) Bayesian inference 

Notes

Acknowledgments

We would like to thank the Laboratory for Retinal Regeneration in RIKEN, Japan, especially for the retinal transplantation team led by Dr. Michiko Mandai. This methodology would not have been developed without the generous support from the team members.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Laboratory for Retinal RegenerationCenter for Biosystems Dynamics Research, RIKENKobeJapan

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