Optical Measurement of Synaptic Vesicle Fusion and Its Inhibition by Inositol Pyrophosphate in Primary Cultured Hippocampal Neurons
Optical molecular and cellular imaging has provided considerable information in various life science research fields. In particular, pHluorin-based assays allow us to specifically investigate presynaptic physiological processes, such as synaptic transmission and synaptic retrieval. Here, I describe quantitative synaptic transmission in primary cultured hippocampal neurons using a vGlut-pHluorin system combined with a high-fidelity optical imaging system and its regulation by inositol pyrophosphate.
Key wordsSynaptic transmission Synaptic vesicle vGlut-pHluorin (vG-pH) Inositol pyrophosphate IP6K
This work was supported by National Research Foundation of Korea Grant 2017R1A2B4007019 funded by the Ministry of Science and Information and Communication Technology.
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